Dr. Florian Dworkowski

Beamline Scientist
photo of Florian Dworkowski
Telefon
+41 56 310 35 84
Paul Scherrer Institut PSI
Forschungsstrasse 111
5232 Villigen PSI
Schweiz
Digitale Visitenkarte

Group Homepage: Macromolecular Crystallography
This page: http://www.psi.ch/people/florian.dworkowski

  • Beamline Instrumentation
  • Serial Crystallography
  • New Method and Software Development
  • in situ Spectroscopy
  • Radiation Damage
  • since 2013 - Beamline Scientist in the Macromolecular Crystallography Group, Swiss Light Source, Switzerland
  • 2010-2012 - PostDoc in the Macromolecular Crystallography Group, Swiss Light Source, Switzerland
  • 2005-2009 - Ph.D. in Biology & Biophysics, Institute for Molecular Biology and Biophysics, Swiss Federal Institute of Technology (ETH), Switzerland
  • 2002-2004 - M.Sc. in Chemistry and Biochemistry, Department for Chemistry and Biochemistry, The University of Oklahoma, USA
  • 2000-2002 - in Chemistry, Department for Chemistry, Technische Universität Berlin, Germany
Google Scholar
ResearcherID D-2807-2011
OrcID 0000-0001-5004-8684
  • Cellini A, Shankar MK, Nimmrich A, Hunt LA, Monrroy L, Mutisya J, et al.
    Directed ultrafast conformational changes accompany electron transfer in a photolyase as resolved by serial crystallography
    Nature Chemistry. 2024; 16: 624-632. https://doi.org/10.1038/s41557-023-01413-9
    DORA PSI
  • Gotthard G, Mous S, Weinert T, Maia RNA, James D, Dworkowski F, et al.
    Capturing the blue-light activated state of the Phot-LOV1 domain from Chlamydomonas reinhardtii using time-resolved serial synchrotron crystallography
    IUCrJ. 2024; 11(5): (17 pp.). https://doi.org/10.1107/S2052252524005608
    DORA PSI
  • Gotthard G, Flores-Ibarra A, Carrillo M, Kepa MW, Mason TJ, Stegmann DP, et al.
    Fixed-target pump–probe SFX: eliminating the scourge of light contamination
    IUCrJ. 2024; 11(5): 1-13. https://doi.org/10.1107/S2052252524005591
    DORA PSI
  • Christou N-E, Apostolopoulou V, Melo DVM, Ruppert M, Fadini A, Henkel A, et al.
    Time-resolved crystallography captures light-driven DNA repair
    Science. 2023; 382(6674): 1015-1020. https://doi.org/10.1126/science.adj4270
    DORA PSI
  • Gruhl T, Weinert T, Rodrigues MJ, Milne CJ, Ortolani G, Nass K, et al.
    Ultrafast structural changes direct the first molecular events of vision
    Nature. 2023; 615: 939-944. https://doi.org/10.1038/s41586-023-05863-6
    DORA PSI
  • Leonarski F, Nan J, Matej Z, Bertrand Q, Furrer A, Gorgisyan I, et al.
    Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source
    IUCrJ. 2023; 10(6): 729-737. https://doi.org/10.1107/S2052252523008618
    DORA PSI
  • Wranik M, Kepa MW, Beale EV, James D, Bertrand Q, Weinert T, et al.
    A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
    Nature Communications. 2023; 14(1): 7956 (12 pp.). https://doi.org/10.1038/s41467-023-43523-5
    DORA PSI
  • Wranik M, Weinert T, Slavov C, Masini T, Furrer A, Gaillard N, et al.
    Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography
    Nature Communications. 2023; 14(1): 903 (12 pp.). https://doi.org/10.1038/s41467-023-36481-5
    DORA PSI
  • Cellini A, Shankar MK, Wahlgren WY, Nimmrich A, Furrer A, James D, et al.
    Structural basis of the radical pair state in photolyases and cryptochromes
    Chemical Communications. 2022; 58(31): 4889-4892. https://doi.org/10.1039/D2CC00376G
    DORA PSI
  • Mous S, Gotthard G, Ehrenberg D, Sen S, Weinert T, Johnson PJM, et al.
    Dynamics and mechanism of a light-driven chloride pump
    Science. 2022; 375(6583): 845-851. https://doi.org/10.1126/science.abj6663
    DORA PSI
  • Nass K, Bacellar C, Cirelli C, Dworkowski F, Gevorkov Y, James D, et al.
    Pink-beam serial femtosecond crystallography for accurate structure-factor determination at an X-ray free-electron laser
    IUCrJ. 2021; 8: 905-920. https://doi.org/10.1107/S2052252521008046
    DORA PSI
  • Lučić M, Chaplin AK, Moreno-Chicano T, Dworkowski FSN, Wilson MT, Svistunenko DA, et al.
    A subtle structural change in the distal haem pocket has a remarkable effect on tuning hydrogen peroxide reactivity in dye decolourising peroxidases from Streptomyces lividans
    Dalton Transactions. 2020; 49(5): 1620-1636. https://doi.org/10.1039/c9dt04583j
    DORA PSI
  • Lučić M, Svistunenko DA, Wilson MT, Chaplin AK, Davy B, Ebrahim A, et al.
    Serial femtosecond zero dose crystallography captures a water-free distal heme site in a dye‐decolourising peroxidase to reveal a catalytic role for an arginine in FeIV=O formation
    Angewandte Chemie International Edition. 2020; 59(48): 21656-21662. https://doi.org/10.1002/anie.202008622
    DORA PSI
  • Nass K, Cheng R, Vera L, Mozzanica A, Redford S, Ozerov D, et al.
    Advances in long-wavelength native phasing at X-ray free-electron lasers
    IUCrJ. 2020; 7: 965-975. https://doi.org/10.1107/S2052252520011379
    DORA PSI
  • Skopintsev P, Ehrenberg D, Weinert T, James D, Kar RK, Johnson PJM, et al.
    Femtosecond-to-millisecond structural changes in a light-driven sodium pump
    Nature. 2020; 583: 314-318. https://doi.org/10.1038/s41586-020-2307-8
    DORA PSI
  • Weinert T, Skopintsev P, James D, Dworkowski F, Panepucci E, Kekilli D, et al.
    Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography
    Science. 2019; 365(6448): 61-65. https://doi.org/10.1126/science.aaw8634
    DORA PSI
  • Horrell S, Kekilli D, Sen K, Owen RL, Dworkowski FSN, Antonyuk SV, et al.
    Enzyme catalysis captured using multiple structures from one crystal at varying temperatures
    IUCrJ. 2018; 5(3): 283-292. https://doi.org/10.1107/S205225251800386X
    DORA PSI
  • Lebugle M, Dworkowski F, Pauluhn A, Guzenko VA, Romano L, Meier N, et al.
    High-intensity x-ray microbeam for macromolecular crystallography using silicon kinoform diffractive lenses
    Applied Optics. 2018; 57(30): 9032-9039. https://doi.org/10.1364/AO.57.009032
    DORA PSI
  • Kekilli D, Petersen CA, Pixton DA, Ghafoor DD, Abdullah GH, Dworkowski FSN, et al.
    Engineering proximal vs. distal heme-NO coordination via dinitrosyl dynamics: implications for NO sensor design
    Chemical Science. 2017; 8(3): 1986-1994. https://doi.org/10.1039/c6sc04190f
    DORA PSI
  • Kekilli D, Moreno-Chicano T, Chaplin AK, Horrell S, Dworkowski FSN, Worrall JAR, et al.
    Photoreduction and validation of haem-ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction
    IUCrJ. 2017; 4: 263-270. https://doi.org/10.1107/S2052252517002159
    DORA PSI
  • Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, et al.
    Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
    Nature Communications. 2017; 8(1): 542 (11 pp.). https://doi.org/10.1038/s41467-017-00630-4
    DORA PSI
  • Jaeger K, Dworkowski F, Nogly P, Milne C, Wang M, Standfuss J
    Serial millisecond crystallography of membrane proteins
    In: Moraes I, ed. The next generation in membrane protein structure determination. Advances in experimental medicine and biology. Cham: Springer; 2016:137-149. https://doi.org/10.1007/978-3-319-35072-1_10
    DORA PSI
  • Botha S, Nass K, Barends T, Kabsch W, Latz B, Dworkowski F, et al.
    Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams
    Acta Crystallographica Section D: Structural Biology. 2015; 71: 387-397. https://doi.org/10.1107/S1399004714026327
    DORA PSI
  • Dworkowski FSN, Hough MA, Pompidor G, Fuchs MR
    Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data
    Acta Crystallographica Section D: Structural Biology. 2015; 71: 27-35. https://doi.org/10.1107/S1399004714015107
    DORA PSI
  • Ghafoor DD, Kekilli D, Abdullah GH, Dworkowski FSN, Hassan HG, Wilson MT, et al.
    Hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal NO adduct in cytochrome c'
    Journal of Biological Inorganic Chemistry. 2015; 20(6): 949-956. https://doi.org/10.1007/s00775-015-1278-y
    DORA PSI
  • Angelaccio S, Dworkowski F, Di Bello A, Milano T, Capitani G, Pascarella S
    Conformational transitions driven by pyridoxal-5′-phosphate uptake in the psychrophilic serine hydroxymethyltransferase from Psychromonas ingrahamii
    Proteins. 2014; 82(10): 2831-2841. https://doi.org/10.1002/prot.24646
    DORA PSI
  • Bolze CS, Helbling RE, Owen RL, Pearson AR, Pompidor G, Dworkowski F, et al.
    Human cellular retinaldehyde-binding protein has secondary thermal 9-cis-retinal isomerase activity
    Journal of the American Chemical Society. 2014; 136(1): 137-146. https://doi.org/10.1021/ja411366w
    DORA PSI
  • Fuchs MR, Pradervand C, Thominet V, Schneider R, Panepucci E, Grunder M, et al.
    D3, the new diffractometer for the macromolecular crystallography beamlines of the Swiss Light Source
    Journal of Synchrotron Radiation. 2014; 21(2): 340-351. https://doi.org/10.1107/S160057751400006X
    DORA PSI
  • Kekilli D, Dworkowski FSN, Pompidor G, Fuchs MR, Andrew CR, Antonyuk S, et al.
    Fingerprinting redox and ligand states in haemprotein crystal structures using resonance Raman spectroscopy
    Acta Crystallographica Section D: Structural Biology. 2014; 70(5): 1289-1296. https://doi.org/10.1107/S1399004714004039
    DORA PSI
  • Merlino A, Fuchs MR, Pica A, Balsamo A, Dworkowski FSN, Pompidor G, et al.
    Selective X-ray-induced NO photodissociation in haemoglobin crystals: evidence from a Raman-assisted crystallographic study
    Acta Crystallographica Section D: Structural Biology. 2013; 69(1): 137-140. https://doi.org/10.1107/S0907444912042229
    DORA PSI
  • Pompidor G, Dworkowski FSN, Thominet V, Schulze-Briese C, Fuchs MR
    A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source
    Journal of Synchrotron Radiation. 2013; 20(5): 765-776. https://doi.org/10.1107/S0909049513016063
    DORA PSI
  • Rajendran C, Dworkowski FSN, Wang M, Schulze-Briese C
    Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector
    Journal of Synchrotron Radiation. 2011; 18(3): 318-328. https://doi.org/10.1107/S090904951100968X
    DORA PSI
  • Eidam O, Dworkowski FSN, Glockshuber R, Grütter MG, Capitani G
    Crystal structure of the ternary FimC-FimFt-FimDN complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD
    FEBS Letters. 2008; 582(5): 651-655. https://doi.org/10.1016/j.febslet.2008.01.030
    DORA PSI