Protocols

Crystallizing membrane proteins using lipidic mesophases
Caffrey M, Cherezov V
Nature Protocols 4, 706–731 (2009).
DOI: 10.1038/nprot.2009.31

Crystallization of Membrane Proteins in Lipidic Mesophases
Liu W, Cherezov V
J. Vis. Exp. 49 (2011).
DOI: 10.3791/2501

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Ishchenko A, Cherezov V, Liu W
J. Vis. Exp. 115 (2016).
DOI: 10.3791/54463

Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser
Nogly P, Weinert T, James D, Carbajo S, Ozerov D, Furrer A, Gashi D, Borin V, Skopintsev P, Jaeger K, Nass K, Båth P, Bosman R, Koglin J, Seaberg M, Lane T, Kekilli D, Brünle S, Tanaka T, Wu W, Milne C, White T, Barty A, Weierstall U, Panneels V, Nango E, Iwata S, Hunter M, Schapiro I, Schertler G, Neutze R, Standfuss J
SCIENCE 361, 145 (2018).
DOI: 10.1126/science.aat0094

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, Gashi D, Vera L, Marsh M, Jaeger K, Dworkowski F, Panepucci E, Basu S, Skopintsev P, Doré Andrew S, Geng T, Cooke Robert M, Liang M, Prota Andrea E, Panneels V, Nogly P, Ermler U, Schertler G, Hennig M, Steinmetz Michel O, Wang M, Standfuss J
NATURE COMMUNICATIONS 8, 542 (2017).
DOI: 10.1038/s41467-017-00630-4

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
Nogly P, Panneels V, Nelson G, Gati C, Kimura T, Milne C, Milathianaki D, Kubo M, Wu W, Conrad C, Coe J, Bean R, Zhao Y, Båth P, Dods R, Harimoorthy R, Beyerlein Kenneth R, Rheinberger J, James D, DePonte D, Li C, Sala L, Williams Garth J, Hunter Mark S, Koglin Jason E, Berntsen P, Nango E, Iwata S, Chapman Henry N, Fromme P, Frank M, Abela R, Boutet S, Barty A, White Thomas A, Weierstall U, Spence J, Neutze R, Schertler G, Standfuss J
NATURE COMMUNICATIONS 7, 12314 (2016).
DOI: 10.1038/ncomms12314

A three-dimensional movie of structural changes in bacteriorhodopsin
Nango E, Royant A, Kubo M, Nakane T, Wickstrand C, Kimura T, Tanaka T, Tono K, Song C, Tanaka R, Arima T, Yamashita A, Kobayashi J, Hosaka T, Mizohata E, Nogly P, Sugahara M, Nam D, Nomura T, Shimamura T, Im D, Fujiwara T, Yamanaka Y, Jeon B, Nishizawa T, Oda K, Fukuda M, Andersson R, Båth P, Dods R, Davidsson J, Matsuoka S, Kawatake S, Murata M, Nureki O, Owada S, Kameshima T, Hatsui T, Joti Y, Schertler G, Yabashi M, Bondar A, Standfuss J, Neutze R, Iwata S
SCIENCE 354 (2016).
DOI: 10.1126/science.aah3497

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
Weierstall U, James D, Wang C, White TA, Wang D, Liu W, Spence JC, Bruce Doak R, Nelson G, Fromme P, Fromme R, Grotjohann I, Kupitz C, Zatsepin NA, Liu H, Basu S, Wacker D, Han GW, Katritch V, Boutet S, Messerschmidt M, Williams GJ, Koglin JE, Marvin Seibert M, Klinker M, Gati C, Shoeman RL, Barty A, Chapman HN, Kirian RA, Beyerlein KR, Stevens RC, Li D, Shah ST, Howe N, Caffrey M, Cherezov V.
NATURE COMMUNICATIONS 5, 3309 (2014).
DOI: 10.1038/ncomms4309

2019

  • Arrestin-1 engineering facilitates complex stabilization with native rhodopsin Haider Raphael S, Wilhelm F, Rizk A, Mutt E, Deupi X, Peterhans C, Mühle J, Berger P, Schertler Gebhard FX, Standfuss J, Ostermaier Martin K
    Scientific Reports 9, 439 (2019).
    DOI: 10.1038/s41598-018-36881-4

2018

  • CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodriguez-Garcia R, Katrukha EA, Weinert T, Olieric V, Kapitein LC, Steinmetz MO, Dogterom M, Akhmanova A
    DEVELOPMENTAL CELL 46, 40 (2018).
  • Crystal structure of rhodopsin in complex with a mini-G o sheds light on the principles of G protein selectivity Tsai C, Pamula F, Nehmé R, Mühle J, Weinert T, Flock T, Nogly P, Edwards Patricia C, Carpenter B, Gruhl T, Ma P, Deupi X, Standfuss J, Tate Christopher G, Schertler Gebhard FX
    Science Advances 4, eaat7052 (2018).
    DOI: 10.1126/sciadv.aat7052
  • In situ serial crystallography for rapid de novo membrane protein structure determination. Huang CY, Olieric V, Howe N, Warshamanage R, Weinert T, Panepucci E, Vogeley L, Basu S, Diederichs K, Caffrey M, Wang M
    Communications Biology 1, 124 (2018).
    DOI: 10.1038/S42003-018-0123-6
  • Ligand channel in pharmacologically stabilized rhodopsin. Mattle D, Kuhn B, Aebi J, Bedoucha M, Kekilli D, Grozinger N, Alker A, Rudolph MG, Schmid G, Schertler GFX, Hennig M, Standfuss J, Dawson RJP
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115, 3640 (2018).
  • Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser Nogly P, Weinert T, James D, Carbajo S, Ozerov D, Furrer A, Gashi D, Borin V, Skopintsev P, Jaeger K, Nass K, Båth P, Bosman R, Koglin J, Seaberg M, Lane T, Kekilli D, Brünle S, Tanaka T, Wu W, Milne C, White T, Barty A, Weierstall U, Panneels V, Nango E, Iwata S, Hunter M, Schapiro I, Schertler G, Neutze R, Standfuss J
    SCIENCE 361, 145 (2018).
    DOI: 10.1126/science.aat0094

2017

  • Perspective: Opportunities for ultrafast science at SwissFEL Abela Rafael, Beaud Paul, van Bokhoven Jeroen A, Chergui Majed, Feurer Thomas, Haase Johannes, Ingold Gerhard, Johnson Steven L, Knopp Gregor, Lemke Henrik, Milne Chris J, Pedrini Bill, Radi Peter, Schertler Gebhard, Standfuss Jorg, Staub Urs, Patthey Luc
    STRUCTURAL DYNAMICS 4, 061602 (2017).
    DOI: 10.1063/1.4997222
  • Role of the nucleotidyl cyclase helical domain in catalytically active dimer formation. Vercellino I, Rezabkova L, Olieric V, Polyhach Y, Weinert T, Kammerer RA, Jeschke G, Korkhov VM
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114, E9821 (2017).
    DOI: 10.1073/PNAS.1712621114
  • Serial crystallography at synchrotrons and X-ray lasers Standfuss J, Spence J
    IUCRJ 4, 100 (2017).
  • Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, Gashi D, Vera L, Marsh M, Jaeger K, Dworkowski F, Panepucci E, Basu S, Skopintsev P, Doré Andrew S, Geng T, Cooke Robert M, Liang M, Prota Andrea E, Panneels V, Nogly P, Ermler U, Schertler G, Hennig M, Steinmetz Michel O, Wang M, Standfuss J
    NATURE COMMUNICATIONS 8, 542 (2017).
    DOI: 10.1038/s41467-017-00630-4
  • Structural biology Signalling under the microscope Tsai ChingJu, Standfuss Joerg, Glaeser Robert M
    NATURE 546, 36-37 (2017).
    DOI: 10.1038/nature22491
  • Structure of the Full-length VEGFR-1 Extracellular Domain in Complex with VEGF-A. Markovic-Mueller S, Stuttfeld E, Asthana M, Weinert T, Bliven S, Goldie KN, Kisko K, Capitani G, Ballmer-Hofer K
    STRUCTURE 25, 341 (2017).
    DOI: 10.1016/J.STR.2016.12.012

2016

  • A three-dimensional movie of structural changes in bacteriorhodopsin Nango Eriko, Royant Antoine, Kubo Minoru, Nakane Takanori, Wickstrand Cecilia, Kimura Tetsunari, Tanaka Tomoyuki, Tono Kensuke, Song Changyong, Tanaka Rie, Arima Toshi, Yamashita Ayumi, Kobayashi Jun, Hosaka Toshiaki, Mizohata Eiichi, Nogly Przemyslaw, Sugahara Michihiro, Nam Daewoong, Nomura Takashi, Shimamura Tatsuro, Im Dohyun, Fujiwara Takaaki, Yamanaka Yasuaki, Jeon Byeonghyun, Nishizawa Tomohiro, Oda Kazumasa, Fukuda Masahiro, Andersson Rebecka, Bath Petra, Dods Robert, Davidsson Jan, Matsuoka Shigeru, Kawatake Satoshi, Murata Michio, Nureki Osamu, Owada Shigeki, Kameshima Takashi, Hatsui Takaki, Joti Yasumasa, Schertler Gebhard, Yabashi Makina, Bondar AnaNicoleta, Standfuss Jorg, Neutze Richard, Iwata So
    SCIENCE 354, 1552-1557 (2016).
    DOI: 10.1126/science.aah3497
  • Data-collection strategy for challenging native SAD phasing Olieric V, Weinert T, Finke Aaron D, Anders C, Li D, Olieric N, Borca Camelia N, Steinmetz Michel O, Caffrey M, Jinek M, Wang M
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72, 421 (2016).
    DOI: 10.1107/S2059798315024110
  • Functional map of arrestin binding to phosphorylated opsin, with and without agonist Peterhans C, Lally Ciara CM, Ostermaier Martin K, Sommer Martha E, Standfuss J
    Scientific Reports 6, 28686 (2016).
    DOI: 10.1038/srep28686
  • Functional map of arrestin binding to phosphorylated opsin, with and without agonist (vol 6, 28686, 2016) Peterhans Christian, Lally Ciara C M, Ostermaier Martin K, Sommer Martha E, Standfuss Jorg
    Scientific Reports 6, 30224 (2016).
    DOI: 10.1038/srep30224
  • In meso in situ In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures Huang ChiaYing, Olieric Vincent, Ma Pikyee, Howe Nicole, Vogeley Lutz, Liu Xiangyu, Warshamanage Rangana, Weinert Tobias, Panepucci Ezequiel, Kobilka Brian, Diederichs Kay, Wang Meitian, Caffrey Martin
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72, 93-112 (2016).
    DOI: 10.1107/S2059798315021683
  • In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures. Huang CY, Olieric V, Ma P, Howe N, Vogeley L, Liu X, Warshamanage R, Weinert T, Panepucci E, Kobilka B, Diederichs K, Wang M, Caffrey M
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 72, 93 (2016).
    DOI: 10.1107/S2059798315021683
  • Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography Nogly P, Panneels V, Nelson G, Gati C, Kimura T, Milne C, Milathianaki D, Kubo M, Wu W, Conrad C, Coe J, Bean R, Zhao Y, Båth P, Dods R, Harimoorthy R, Beyerlein Kenneth R, Rheinberger J, James D, DePonte D, Li C, Sala L, Williams Garth J, Hunter Mark S, Koglin Jason E, Berntsen P, Nango E, Iwata S, Chapman Henry N, Fromme P, Frank M, Abela R, Boutet S, Barty A, White Thomas A, Weierstall U, Spence J, Neutze R, Schertler G, Standfuss J
    NATURE COMMUNICATIONS 7, 12314 (2016).
    DOI: 10.1038/ncomms12314
  • Serial Millisecond Crystallography of Membrane Proteins Jaeger K, Dworkowski F, Nogly P, Milne C, Wang M, Standfuss J
    ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY 922, 137 (2016).
    DOI: 10.1007/978-3-319-35072-1_10
  • Structural role of the T94I rhodopsin mutation in congenital stationary night blindness Singhal Ankita, Guo Ying, Matkovic Milos, Schertler Gebhard, Deupi Xavier, Yan Elsa C Y, Standfuss Joerg
    EMBO REPORTS 17, 1431-1440 (2016).
    DOI: 10.15252/embr.201642671