Cellular structural biology and imaging

Using core stengths in electron tomography and protein bioengineering the cellular structural biology  group focuses on analysis of 3D structures of biological macromolecules in the cell, in particular using cryo-electron tomography.

Our Research

Our focus is electron and X-ray imaging and related methodological development. Takashi Ishikawa is interested in eukaryotic cilia/flagella, which are microtubule-based organelles and enable cellular motility, extracellular flow as well as sensing. His group pursues 3D imaging of motor, regulatory, and cytoskeletal proteins, intact cilia and ciliated cells and tissues, employing single particle cryo-EM, cryo-electron tomography and ptychographic X-ray tomography. Their aim is to reveal molecular mechanism of ciliary function. The Benoit group is interested in molecular and cellular structures of membrane proteins, especially receptors. They use single particle cryo-EM to analyze molecular structure of membrane proteins, developing related genetic engineering techniques. 

for more information please contact:

Takashi Ishikawa, Group leader
Cellular and molecular structural biology on eukaryotic cilia and flagella

Roger Benoit, Scientist
Engineered scaffolds for protein structure elucidation by cryo-EM and crystallography
 

Tobias Bierig Ph.D. Student
Gabriella Collu Ph.D. Student
Khanh Huy Bui Postdoc
Aditi Maheshwari Ph.D. Student
Malkova Barbora Postdoc
Akira Noga Postdoc
Tandis Movassagh Ph.D. Student
Jagan Mohan Obbineni Ph.D. Student
Gaia Pigino Postdoc
Emiliya Poghosyan Ph.D. Student
Farooque Razvi Shaik Postdoc
Iman Rostami Postdoc
Sarah Shahmoradian Scientist
Hung Tri Tran Ph.D. Student
Hironori Ueno Postdoc
Valtteri Järvinen Ph.D. Student

 

Publications

Group publications since 2010

 

  • Christen P, Jaussi R, Benoit R
    Biochemie und Molekularbiologie. Eine Einführung in 40 Lerneinheiten
    2nd ed. Berlin: Springer Nature; 2024. https://doi.org/10.1007/978-3-662-65477-4
    DORA PSI
  • Wang J, Beyer D, Vaccarin C, He Y, Tanriver M, Benoit R, et al.
    Development of radiofluorinated MLN-4760 derivatives for PET imaging of the SARS-CoV-2 entry receptor ACE2
    European Journal of Nuclear Medicine and Molecular Imaging. 2024; 52: 9-21. https://doi.org/10.1007/s00259-024-06831-6
    DORA PSI
  • Zimmermann N, Ishikawa T
    Comparative structural study on axonemal and cytoplasmic dyneins
    Cytoskeleton. 2024; 81(11): 681-690. https://doi.org/10.1002/cm.21897
    DORA PSI
  • Behbahanipour M, Benoit R, Navarro S, Ventura S
    OligoBinders: bioengineered soluble amyloid-like nanoparticles to bind and neutralize SARS-CoV-2
    ACS Applied Materials and Interfaces. 2023; 15(9): 11444-11457. https://doi.org/10.1021/acsami.2c18305
    DORA PSI
  • Cvjetan N, Schuler LD, Ishikawa T, Walde P
    Optimization and enhancement of the peroxidase-like activity of hemin in aqueous solutions of sodium dodecylsulfate
    ACS Omega. 2023; 8(45): 42878-42899. https://doi.org/10.1021/acsomega.3c05915
    DORA PSI
  • Farnung J, Muhar M, Liang JR, Tolmachova KA, Benoit RM, Corn JE, et al.
    Semisynthetic LC3 probes for autophagy pathways reveal a noncanonical LC3 interacting region motif crucial for the enzymatic activity of human ATG3
    ACS Central Science. 2023; 9(5): 1025-1034. https://doi.org/10.1021/acscentsci.3c00009
    DORA PSI
  • Ishikawa T
    Architecture of intraflagellar transport complexes
    Nature Structural and Molecular Biology. 2023; 30(5): 570-573. https://doi.org/10.1038/s41594-023-00986-w
    DORA PSI
  • Ishikawa T
    Cryo-electron tomography
    In: Bradshaw RA, Hart GW, Stahl PD, eds. Organizational aspects of cell biology - part 1. Encyclopedia of cell biology. Amsterdam: Elsevier; 2023:28-36. https://doi.org/10.1016/B978-0-12-821618-7.00084-5
    DORA PSI
  • Ishikawa T
    Mass-spec, cryo-EM and AI join forces for a close look at the transporter complex in cilia
    EMBO Journal. 2023; 42: e113010 (3 pp.). https://doi.org/10.15252/embj.2022113010
    DORA PSI
  • Yildiz A, Ishikawa T
    Dyneins
    In: Bradshaw RA, Hart GW, Stahl PD, eds. Organizational aspects of cell biology - part 2. Encyclopedia of cell biology. Amsterdam: Elsevier; 2023:110-137. https://doi.org/10.1016/B978-0-12-821618-7.00094-8
    DORA PSI
  • Zimmermann N, Noga A, Obbineni JM, Ishikawa T
    ATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography
    EMBO Journal. 2023; 42(12): e112466 (15 pp.). https://doi.org/10.15252/embj.2022112466
    DORA PSI
  • Collu G, Bierig T, Krebs A-S, Engilberge S, Varma N, Guixà-González R, et al.
    Chimeric single α-helical domains as rigid fusion protein connections for protein nanotechnology and structural biology
    Structure. 2022; 30(1): 95-106. https://doi.org/10.1016/j.str.2021.09.002
    DORA PSI
  • Ishikawa T
    Structure of motile cilia
    In: Harris RJ, Marles-Wright J, eds. Macromolecular protein complexes IV. Structure and function. Subcellular biochemistry. Cham: Springer Nature; 2022:471-494. https://doi.org/10.1007/978-3-031-00793-4_15
    DORA PSI
  • Noga A, Horii M, Goto Y, Toyooka K, Ishikawa T, Hirono M
    Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel
    EMBO Journal. 2022; 41(20): e104582 (14 pp.). https://doi.org/10.15252/embj.2020104582
    DORA PSI
  • Galaz-Montoya JG, Shahmoradian SH, Shen K, Frydman J, Chiu W
    Cryo-electron tomography provides topological insights into mutant huntingtin exon 1 and polyQ aggregates
    Communications Biology. 2021; 4(1): 849 (9 pp.). https://doi.org/10.1038/s42003-021-02360-2
    DORA PSI
  • Kutomi O, Yamamoto R, Hirose K, Mizuno K, Nakagiri Y, Imai H, et al.
    A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light
    Science Advances. 2021; 7(9): eabf3621 (12 pp.). https://doi.org/10.1126/sciadv.abf3621
    DORA PSI
  • Panneels V, Diaz A, Imsand C, Guizar-Sicairos M, Müller E, Bittermann AG, et al.
    Imaging of retina cellular and subcellular structures using ptychographic hard X-ray tomography
    Journal of Cell Science. 2021; 134(19): jcs258561 (8 pp.). https://doi.org/10.1242/jcs.258561
    DORA PSI
  • Tran HT, Lucas MS, Ishikawa T, Shahmoradian SH, Padeste C
    A compartmentalized neuronal cell-culture platform compatible with cryo-fixation by high-pressure freezing for ultrastructural imaging
    Frontiers in Neuroscience. 2021; 15: 726763 (15 pp.). https://doi.org/10.3389/fnins.2021.726763
    DORA PSI
  • Yamamoto R, Hwang J, Ishikawa T, Kon T, Sale WS
    Composition and function of ciliary inner-dynein-arm subunits studied in Chlamydomonas reinhardtii
    Cytoskeleton. 2021; 78(3): 77-96. https://doi.org/10.1002/cm.21662
    DORA PSI
  • Bierig T, Collu G, Blanc A, Poghosyan E, Benoit RM
    Design, expression, purification, and characterization of a YFP-tagged 2019-n CoV spike receptor-binding domain construct
    Frontiers in Bioengineering and Biotechnology. 2020; 8: 618615 (10 pp.). https://doi.org/10.3389/fbioe.2020.618615
    DORA PSI
  • Holler M, Ihli J, Tsai EHR, Nudelman F, Verezhak M, van de Berg WDJ, et al.
    A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures
    Journal of Synchrotron Radiation. 2020; 27(2): 472-476. https://doi.org/10.1107/S1600577519017028
    DORA PSI
  • Poghosyan E, Iacovache I, Faltova L, Leitner A, Yang P, Diener DR, et al.
    The structure and symmetry of the radial spoke protein complex in Chlamydomonas flagella
    Journal of Cell Science. 2020; 133(16): jcs245233 (9 pp.). https://doi.org/10.1242/jcs.245233
    DORA PSI
  • Rösner B, Finizio S, Koch F, Döring F, Guzenko VA, Langer M, et al.
    Soft x-ray microscopy with 7 nm resolution
    Optica. 2020; 7(11): 1602-1608. https://doi.org/10.1364/OPTICA.399885
    DORA PSI
  • Skopintsev P, Ehrenberg D, Weinert T, James D, Kar RK, Johnson PJM, et al.
    Femtosecond-to-millisecond structural changes in a light-driven sodium pump
    Nature. 2020; 583: 314-318. https://doi.org/10.1038/s41586-020-2307-8
    DORA PSI
  • Tran HT, Tsai EHR, Lewis AJ, Moors T, Bol JGJM, Rostami I, et al.
    Alterations in sub-axonal architecture between normal aging and Parkinson's diseased human brains using label-free cryogenic X-ray nanotomography
    Frontiers in Neuroscience. 2020; 14: 570019 (22 pp.). https://doi.org/10.3389/fnins.2020.570019
    DORA PSI
  • Guerrero-Ferreira RC, Hupfeld M, Nazarov S, Taylor NMI, Shneider MM, Obbineni JM, et al.
    Structure and transformation of bacteriophage A511 baseplate and tail upon infection of Listeria cells
    EMBO Journal. 2019; 38(3): e99455 (20 pp.). https://doi.org/10.15252/embj.201899455
    DORA PSI
  • Kashima K, Fujisaki T, Serrano-Luginbühl S, Kissner R, Janošević Ležaić A, Bajuk-Bogdanović D, et al.
    Effect of template type on the Trametes versicolor laccase-catalyzed oligomerization of the aniline dimer p-aminodiphenylamine (PADPA)
    ACS Omega. 2019; 4(2): 2931-2947. https://doi.org/10.1021/acsomega.8b03441
    DORA PSI
  • Krebs A-S, Bierig T, Collu G, Benoit RM
    Seamless insert-plasmid assembly at sub-terminal homologous sequences
    Plasmid. 2019; 106: 102445 (9 pp.). https://doi.org/10.1016/j.plasmid.2019.102445
    DORA PSI
  • Lewis AJ, Genoud C, Pont M, van de Berg WDJ, Frank S, Stahlberg H, et al.
    Imaging of post-mortem human brain tissue using electron and X-ray microscopy
    Current Opinion in Structural Biology. 2019; 58: 138-148. https://doi.org/10.1016/j.sbi.2019.06.003
    DORA PSI
  • Li T, Krumeich F, Ihli J, Ma Z, Ishikawa T, Pinar AB, et al.
    Heavy atom labeling enables silanol defect visualization in silicalite-1 crystals
    Chemical Communications. 2019; 55(4): 482-485. https://doi.org/10.1039/c8cc07912a
    DORA PSI
  • Rostami I, Alanagh HR, Hu Z, Shahmoradian SH
    Breakthroughs in medicine and bioimaging with up-conversion nanoparticles
    International Journal of Nanomedicine. 2019; 14: 7759-7780. https://doi.org/10.2147/IJN.S221433
    DORA PSI
  • Shahmoradian SH, Lewis AJ, Genoud C, Hench J, Moors T, Navarro PP, et al.
    Lewy pathology in Parkinson’s disease consists of crowded organelles and lipid membranes
    Nature Neuroscience. 2019; 22(7): 1099-1109. https://doi.org/10.1038/s41593-019-0423-2
    DORA PSI
  • Zhu X, Poghosyan E, Rezabkova L, Mehall B, Sakakibara H, Hirono M, et al.
    The roles of a flagellar HSP40 ensuring rhythmic beating
    Molecular Biology of the Cell. 2019; 30(2): 228-241. https://doi.org/10.1091/mbc.E18-01-0047
    DORA PSI
  • Benoit RM
    Botulinum neurotoxin diversity from a gene-centered view
    Toxins. 2018; 10(8): 310 (14 pp.). https://doi.org/10.3390/toxins10080310
    DORA PSI
  • Holler M, Raabe J, Diaz A, Guizar-Sicairos M, Wepf R, Odstrcil M, et al.
    OMNY – a tOMography Nano crYo stage
    Review of Scientific Instruments. 2018; 89(4): 043706 (13 pp.). https://doi.org/10.1063/1.5020247
    DORA PSI
  • Isabettini S, Stucki S, Massabni S, Baumgartner ME, Reckey PQ, Kohlbrecher J, et al.
    Development of smart optical gels with highly magnetically responsive bicelles
    ACS Applied Materials and Interfaces. 2018; 10(10): 8926-8936. https://doi.org/10.1021/acsami.7b17134
    DORA PSI
  • Navarro PP, Genoud C, Castaño-Díez D, Graff-Meyer A, Lewis AJ, de Gier Y, et al.
    Cerebral Corpora amylacea are dense membranous labyrinths containing structurally preserved cell organelles
    Scientific Reports. 2018; 8(1): 18046 (13 pp.). https://doi.org/10.1038/s41598-018-36223-4
    DORA PSI
  • Neuhaus F, Mueller D, Tanasescu R, Stefaniu C, Zaffalon P-L, Balog S, et al.
    Against the rules: pressure induced transition from high to reduced order
    Soft Matter. 2018; 14(19): 3978-3986. https://doi.org/10.1039/c8sm00212f
    DORA PSI
  • Neuhaus F, Mueller D, Tanasescu R, Balog S, Ishikawa T, Brezesinski G, et al.
    Synthesis and biophysical characterization of an odd-numbered 1,3-diamidophospholipid
    Langmuir. 2018; 34(10): 3215-3220. https://doi.org/10.1021/acs.langmuir.7b04227
    DORA PSI
  • Benoit RM, Schärer MA, Wieser MM, Li X, Frey D, Kammerer RA
    Crystal structure of the BoNT/A2 receptor-binding domain in complex with the luminal domain of its neuronal receptor SV2C
    Scientific Reports. 2017; 7: 43588 (7 pp.). https://doi.org/10.1038/srep43588
    DORA PSI
  • Buscema M, Matviykiv S, Mészáros T, Gerganova G, Weinberger A, Mettal U, et al.
    Immunological response to nitroglycerin-loaded shear-responsive liposomes in vitro and in vivo
    Journal of Controlled Release. 2017; 264: 14-23. https://doi.org/10.1016/j.jconrel.2017.08.010
    DORA PSI
  • Heydenreich FM, Miljuš T, Jaussi R, Benoit R, Milić D, Veprintsev DB
    High-throughput mutagenesis using a two-fragment PCR approach
    Scientific Reports. 2017; 7: 6787 (11 pp.). https://doi.org/10.1038/s41598-017-07010-4
    DORA PSI
  • Holler M, Raabe J, Wepf R, Shahmoradian SH, Diaz A, Sarafimov B, et al.
    OMNY PIN - a versatile sample holder for tomographic measurements at room and cryogenic temperatures
    Review of Scientific Instruments. 2017; 88(11): 113701 (9 pp.). https://doi.org/10.1063/1.4996092
    DORA PSI
  • Isabettini S, Liebi M, Kohlbrecher J, Ishikawa T, Fischer P, Windhab EJ, et al.
    Mastering the magnetic susceptibility of magnetically responsive bicelles with 3β-amino-5-cholestene and complexed lanthanide ions
    Physical Chemistry Chemical Physics. 2017; 19(17): 10820-10824. https://doi.org/10.1039/c7cp01025g
    DORA PSI
  • Isabettini S, Baumgartner ME, Reckey PQ, Kohlbrecher J, Ishikawa T, Fischer P, et al.
    Methods for generating highly magnetically responsive lanthanide-chelating phospholipid polymolecular assemblies
    Langmuir. 2017; 33(25): 6363-6371. https://doi.org/10.1021/acs.langmuir.7b00725
    DORA PSI
  • Isabettini S, Massabni S, Hodzic A, Durovic D, Kohlbrecher J, Ishikawa T, et al.
    Molecular engineering of lanthanide ion chelating phospholipids generating assemblies with a switched magnetic susceptibility
    Physical Chemistry Chemical Physics. 2017; 19(31): 20991-21002. https://doi.org/10.1039/c7cp03994h
    DORA PSI
  • Ishikawa T
    Axoneme structure from motile cilia
    Cold Spring Harbor Perspectives in Biology. 2017; 9(1): a028076 (18 pp.). https://doi.org/10.1101/cshperspect.a028076
    DORA PSI
  • Neuhaus F, Mueller D, Tanasescu R, Balog S, Ishikawa T, Brezesinski G, et al.
    Vesicle origami: cuboid phospholipid vesicles formed by template-free self-assembly
    Angewandte Chemie International Edition. 2017; 56(23): 6515-6518. https://doi.org/10.1002/anie.201701634
    DORA PSI
  • Obbineni JM, Yamamoto R, Ishikawa T
    A simple and fast approach for missing-wedge invariant classification of subtomograms extracted from filamentous structures
    Journal of Structural Biology. 2017; 197(2): 145-154. https://doi.org/10.1016/j.jsb.2016.08.003
    DORA PSI
  • Shahmoradian SH, Tsai EHR, Diaz A, Guizar-Sicairos M, Raabe J, Spycher L, et al.
    Three-dimensional imaging of biological tissue by cryo X-ray ptychography
    Scientific Reports. 2017; 7: 6291 (12 pp.). https://doi.org/10.1038/s41598-017-05587-4
    DORA PSI
  • Yamamoto R, Obbineni JM, Alford LM, Ide T, Owa M, Hwang J, et al.
    Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms
    PLoS Genetics. 2017; 13(10): e1006996 (21 pp.). https://doi.org/10.1371/journal.pgen.1006996
    DORA PSI
  • Zhu X, Poghosyan E, Gopal R, Liu Y, Ciruelas KS, Maizy Y, et al.
    General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase
    Molecular Biology of the Cell. 2017; 28(22): 3029-3042. https://doi.org/10.1091/mbc.E17-03-0156
    DORA PSI
  • Aroua S, Tiu EGV, Ishikawa T, Yamakoshi Y
    Well-defined amphiphilic C60-PEG conjugates: water-soluble and thermoresponsive materials
    Helvetica Chimica Acta. 2016; 99(10): 805-813. https://doi.org/10.1002/hlca.201600171
    DORA PSI
  • Benoit RM, Ostermeier C, Geiser M, Li JSZ, Widmer H, Auer M
    Seamless insert-plasmid assembly at high efficiency and low cost
    PLoS One. 2016; 11(4): e0153158 (13 pp.). https://doi.org/10.1371/journal.pone.0153158
    DORA PSI
  • Bianchi S, van Riel WE, Kraatz SHW, Olieric N, Frey D, Katrukha EA, et al.
    Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations
    Scientific Reports. 2016; 6: 30668 (16 pp.). https://doi.org/10.1038/srep30668
    DORA PSI
  • Christen P, Jaussi R, Benoit R
    Biochemie und Molekularbiologie. Eine Einführung in 40 Lerneinheiten
    Berlin, Heidelberg: Springer; 2016. https://doi.org/10.1007/978-3-662-46430-4
    DORA PSI
  • Isabettini S, Liebi M, Kohlbrecher J, Ishikawa T, Windhab EJ, Fischer P, et al.
    Tailoring bicelle morphology and thermal stability with lanthanide-chelating cholesterol conjugates
    Langmuir. 2016; 32(35): 9005-9014. https://doi.org/10.1021/acs.langmuir.6b01968
    DORA PSI
  • Pfister B, Sánchez-Ferrer A, Diaz A, Lu K, Otto C, Holler M, et al.
    Recreating the synthesis of starch granules in yeast
    eLife. 2016; 5: e15552 (29 pp.). https://doi.org/10.7554/eLife.15552
    DORA PSI
  • Shen K, Calamini B, Fauerbach JA, Ma B, Shahmoradian SH, Serrano Lachapel IL, et al.
    Control of the structural landscape and neuronal proteotoxicity of mutant Huntingtin by domains flanking the polyQ tract
    eLife. 2016; 5: e18065 (29 pp.). https://doi.org/10.7554/eLife.18065
    DORA PSI
  • Tanasescu R, Lanz MA, Mueller D, Tassler S, Ishikawa T, Reiter R, et al.
    Vesicle origami and the influence of cholesterol on lipid packing
    Langmuir. 2016; 32(19): 4896-4903. https://doi.org/10.1021/acs.langmuir.6b01143
    DORA PSI
  • Aroua S, Tiu EGV, Ayer M, Ishikawa T, Yamakoshi Y
    RAFT synthesis of poly(vinylpyrrolidone) amine and preparation of a water-soluble C60-PVP conjugate
    Polymer Chemistry. 2015; 6(14): 2616-2619. https://doi.org/10.1039/c4py01333f
    DORA PSI
  • Benoit RM, Frey D, Wieser MM, Thieltges KM, Jaussi R, Capitani G, et al.
    Structure of the BoNT/A1 - Receptor complex
    Toxicon. 2015; 107(Part A): 25-31. https://doi.org/10.1016/j.toxicon.2015.08.002
    DORA PSI
  • Diaz A, Malkova B, Holler M, Guizar-Sicairos M, Lima E, Panneels V, et al.
    Three-dimensional mass density mapping of cellular ultrastructure by ptychographic X-ray nanotomography
    Journal of Structural Biology. 2015; 192(3): 461-469. https://doi.org/10.1016/j.jsb.2015.10.008
    DORA PSI
  • Fodor D, Ishikawa T, Krumeich F, van Bokhoven JA
    Synthesis of single crystal nanoreactor materials with multiple catalytic functions by incipient wetness impregnation and ion exchange
    Advanced Materials. 2015; 27(11): 1919-1923. https://doi.org/10.1002/adma.201404628
    DORA PSI
  • Geertsma ER, Chang Y-N, Shaik FR, Neldner Y, Pardon E, Steyaert J, et al.
    Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family
    Nature Structural and Molecular Biology. 2015; 22(10): 803-808. https://doi.org/10.1038/nsmb.3091
    DORA PSI
  • Ishikawa T
    Cryo-electron tomography of motile cilia and flagella
    Cilia. 2015; 4(Suppl. 1): 3 (20 pp.). https://doi.org/10.1186/s13630-014-0012-7
    DORA PSI
  • Liebi M, Kuster S, Kohlbrecher J, Ishikawa T, Walde P, Windhab EJ, et al.
    Design of magnetically responsive phospholipid bicelles towards switchable optical hydrogels
    Swiss Neutron News. 2015:4-13.
    DORA PSI
  • Maheshwari A, Obbineni JM, Bui KH, Shibata K, Toyoshima YY, Ishikawa T
    α- and β-Tubulin Lattice of the Axonemal Microtubule Doublet and Binding Proteins Revealed by Single Particle Cryo-Electron Microscopy and Tomography
    Structure. 2015; 23(9): 1584-1595. https://doi.org/10.1016/j.str.2015.06.017
    DORA PSI
  • Weinberger A, Tanasescu R, Stefaniu C, Fedotenko LA, Favarger F, Ishikawa T, et al.
    Bilayer properties of 1,3-diamidophospholipids
    Langmuir. 2015; 31(6): 1879-1884. https://doi.org/10.1021/la5041745
    DORA PSI
  • Benoit RM, Frey D, Hilbert M, Kevenaar JT, Wieser MM, Stirnimann CU, et al.
    Structural basis for recognition of synaptic vesicle protein 2C by botulinum neurotoxin A
    Nature. 2014; 505(7481): 108-111. https://doi.org/10.1038/nature12732
    DORA PSI
  • Ishikawa T
    Protein tagging reveals new insights into signaling in flagella
    Journal of Cell Biology. 2014; 204(5): 631-633. https://doi.org/10.1083/jcb.201401142
    DORA PSI
  • Kammerer RA, Benoit RM
    Botulinum neurotoxins: new questions arising from structural biology
    Trends in Biochemical Sciences. 2014; 39(11): 517-526. https://doi.org/10.1016/j.tibs.2014.08.009
    DORA PSI
  • Liebi M, Kuster S, Kohlbrecher J, Ishikawa T, Fischer P, Walde P, et al.
    Magnetically enhanced bicelles delivering switchable anisotropy in optical gels
    ACS Applied Materials and Interfaces. 2014; 6(2): 1100-1105. https://doi.org/10.1021/am4046469
    DORA PSI
  • Ueno H, Bui KH, Ishikawa T, Imai Y, Yamaguchi T, Ishikawa T
    Structure of dimeric axonemal dynein in cilia suggests an alternative mechanism of force generation
    Cytoskeleton. 2014; 71(7): 412-422. https://doi.org/10.1002/cm.21180
    DORA PSI
  • Bui KH, Ishikawa T
    3D structural analysis of flagella/cilia by cryo-electron tomography
    In: Marshall WF, ed. Cilia, part A. Methods in enzymology. Amsterdam: Elsevier; 2013:305-323. https://doi.org/10.1016/B978-0-12-397945-2.00017-2
    DORA PSI
  • Ishikawa T
    3D structure of eukaryotic flagella/cilia by cryo-electron tomography
    Biophysics. 2013; 9: 141-148. https://doi.org/10.2142/biophysics.9.141
    DORA PSI
  • Liebi M, Kuster S, Kohlbrecher J, Ishikawa T, Fischer P, Walde P, et al.
    Cholesterol-diethylenetriaminepentaacetate complexed with thulium ions integrated into bicelles to increase their magnetic alignability
    Journal of Physical Chemistry B. 2013; 117(47): 14743-14748. https://doi.org/10.1021/jp406599c
    DORA PSI
  • Bui KH, Yagi T, Yamamoto R, Kamiya R, Ishikawa T
    Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme
    Journal of Cell Biology. 2012; 198(5): 913-925. https://doi.org/10.1083/jcb.201201120
    DORA PSI
  • Ishikawa T
    Structural biology of cytoplasmic and axonemal dyneins
    Journal of Structural Biology. 2012; 179(2): 229-234. https://doi.org/10.1016/j.jsb.2012.05.016
    DORA PSI
  • Junker K, Zandomeneghi G, Guo Z, Kissner R, Ishikawa T, Kohlbrecher J, et al.
    Mechanistic aspects of the horseradish peroxidase-catalysed polymerisation of aniline in the presence of AOT vesicles as templates
    RSC Advances. 2012; 2(16): 6478-6495. https://doi.org/10.1039/c2ra20566a
    DORA PSI
  • Liebi M, Kohlbrecher J, Ishikawa T, Fischer P, Walde P, Windhab EJ
    Cholesterol increases the magnetic aligning of bicellar disks from an aqueous mixture of DMPC and DMPE-DTPA with complexed thulium ions
    Langmuir. 2012; 28(29): 10905-10915. https://doi.org/10.1021/la3019327
    DORA PSI
  • Lowell AN, Qiao H, Liu T, Ishikawa T, Zhang H, Oriana S, et al.
    Functionalized low-density lipoprotein nanoparticles for in vivo enhancement of atherosclerosis on magnetic resonance images
    Bioconjugate Chemistry. 2012; 23(11): 2313-2319. https://doi.org/10.1021/bc300561e
    DORA PSI
  • Maheshwari A, Ishikawa T
    Heterogeneity of dynein structure implies coordinated suppression of dynein motor activity in the axoneme
    Journal of Structural Biology. 2012; 179(2): 235-241. https://doi.org/10.1016/j.jsb.2012.04.018
    DORA PSI
  • Pigino G, Maheshwari A, Bui KH, Shingyoji C, Kamimura S, Ishikawa T
    Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins
    Journal of Structural Biology. 2012; 178(2): 199-206. https://doi.org/10.1016/j.jsb.2012.02.012
    DORA PSI
  • Tokutsu R, Kato N, Bui KH, Ishikawa T, Minagawa J
    Revisiting the supramolecular organization of photosystem II in Chlamydomonas reinhardtii
    Journal of Biological Chemistry. 2012; 287(37): 31574-31581. https://doi.org/10.1074/jbc.M111.331991
    DORA PSI
  • Ueno H, Ishikawa T, Bui KH, Gonda K, Ishikawa T, Yamaguchi T
    Analysis of ciliary motion and the axonemal structure in the mouse respiratory cilia
    In: ASME 2012 summer bioengineering conference, SBC 2012. ; 2012:(2 pp.). https://doi.org/10.1115/SBC2012-80232
    DORA PSI
  • Ueno H, Ishikawa T, Bui KH, Gonda K, Ishikawa T, Yamaguchi T
    Mouse respiratory cilia with the asymmetric axonemal structure on sparsely distributed ciliary cells can generate overall directional flow
    Nanomedicine: Nanotechnology, Biology and Medicine. 2012; 8(7): 1081-1087. https://doi.org/10.1016/j.nano.2012.01.004
    DORA PSI
  • Bui KH, Pigino G, Ishikawa T
    Three-dimensional structural analysis of eukaryotic flagella/cilia by electron cryo-tomography
    Journal of Synchrotron Radiation. 2011; 18(1): 2-5. https://doi.org/10.1107/S0909049510036812
    DORA PSI
  • Ishikawa T
    3D structures of axonemes
    In: Hirose K, ed. Handbook of Dynein. New York: Jenny Stanford Publishing; 2011:245-266. https://doi.org/10.1201/b11622
    DORA PSI
  • Ishikawa T
    Organization of dyneins in the axoneme
    In: King SM, ed. Dyneins. Structure, biology and disease. Elsevier; 2011:244-271. https://doi.org/10.1016/B978-0-12-382004-4.10008-1
    DORA PSI
  • Pigino G, Bui KH, Maheshwari A, Lupetti P, Diener D, Ishikawa T
    Cryoelectron tomography of radial spokes in cilia and flagella
    Journal of Cell Biology. 2011; 195(4): 673-687. https://doi.org/10.1083/jcb.201106125
    DORA PSI
  • Benoit RM, Meisner N-C, Kallen J, Graff P, Hemmig R, Cèbe R, et al.
    The X-ray crystal structure of the first RNA recognition motif and site-directed mutagenesis suggest a possible hur redox sensing mechanism
    Journal of Molecular Biology. 2010; 397(5): 1231-1244. https://doi.org/10.1016/j.jmb.2010.02.043
    DORA PSI

Lab. of Nanoscale Biology
Paul Scherrer Institute PSI
Forschungsstrasse 111
5232 Villigen PSI, Switzerland