We have a long-lasting interest in receptor trafficking and signaling. In order to measure trafficking and signaling of receptors, we developed several tools that are now widely used.
MultiLabel/ MultiPrime: This is a modular, cre/LoxP-based multigene expression system for mammalian cells.
Squassh/ SquasshAnalyst: These tools allow the segmentation of fluorescence images and the quantification of colocalization between different channels (Rizk et al., 2014; Rizk et al., 2015).
GPCR activation assays: These assays are based on split nanoluciferase. They are available for several arrestins (Spillmann et al., 2020) and for GRK-2/ -3/ -5 (Romantini et al., 2021).