Biography
John received a BSc in Biochemistry from Imperial College London, UK before completing a DPhil in Biochemistry at the University of Oxford, UK in membrane protein structural biology under the guidance of Prof. Simon Newstead. In 2016, John moved to the UK synchrotron facility, Diamond Light Source, where he developed serial crystallography techniques on beamline I24. John was then awarded a PSI-Marie Skłodowska Curie Fellowship in the MX group at PSI in 2019 to continue these investigations. In 2020, John became the serial femtosecond crystallography (SFX) beamline scientist on Cristallina, the third experimental station of SwissFEL's ARAMIS beamline.
Scientific Research
Since 2018, John has tried to develop strategies to create the samples required for modern serial crystallography experiments at either synchrotrons or XFELs [Beale et al., (2019), Beale et al., (2021)]. These include methods to create large volumes of micro-crystals and fixed-target mounting and loading techniques [Martiel et al., (2021), Carrillo et alI., (2023)]. Currently, as leader of the SwissMX project, John is primarily responsible for the delivery and support of user beamtime and the development of instrumentation. This responsibility significantly directs John's research towards commissioning and developing instrumentation for user experiments [Gotthard et al., (2024)].
Institutional Responsibilities
Since September 2020, John has been the leader of the Cristallina-MX project which delivery SFX and SFX pump-probe beamtime for SwissFEL users at the Cristallina experimental station. Cristallina has been in user operation since January 2024 and from 2025 will offer fixed-target and high-viscosity-extruder (HVE) based sample delivery. For the latest information, please visit the Cristallina-MX website.
Publications
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Gotthard G, Flores-Ibarra A, Carrillo M, Kepa MW, Mason TJ, Stegmann DP, et al.
Fixed-target pump–probe SFX: eliminating the scourge of light contamination
IUCrJ. 2024; 11(5): 1-13. https://doi.org/10.1107/S2052252524005591
DORA PSI -
Warren AJ, Trincao J, Crawshaw AD, Beale EV, Duller G, Stallwood A, et al.
VMXm – A sub-micron focus macromolecular crystallography beamline at Diamond Light Source
Journal of Synchrotron Radiation. 2024; 31(Pt 6): 1593-1608. https://doi.org/10.1107/S1600577524009160
DORA PSI -
Carrillo M, Mason TJ, Karpik A, Martiel I, Kepa MW, McAuley KE, et al.
Micro-structured polymer fixed targets for serial crystallography at synchrotrons and XFELs
IUCrJ. 2023; 10(6): 678-693. https://doi.org/10.1107/S2052252523007595
DORA PSI -
Christou N-E, Apostolopoulou V, Melo DVM, Ruppert M, Fadini A, Henkel A, et al.
Time-resolved crystallography captures light-driven DNA repair
Science. 2023; 382(6674): 1015-1020. https://doi.org/10.1126/science.adj4270
DORA PSI -
Owen RL, de Sanctis D, Pearson AR, Beale JH
A standard descriptor for fixed-target serial crystallography
Acta Crystallographica Section D: Structural Biology. 2023; 79(8): 668-672. https://doi.org/10.1107/S2059798323005429
DORA PSI -
Wranik M, Kepa MW, Beale EV, James D, Bertrand Q, Weinert T, et al.
A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
Nature Communications. 2023; 14(1): 7956 (12 pp.). https://doi.org/10.1038/s41467-023-43523-5
DORA PSI -
Wranik M, Weinert T, Slavov C, Masini T, Furrer A, Gaillard N, et al.
Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography
Nature Communications. 2023; 14(1): 903 (12 pp.). https://doi.org/10.1038/s41467-023-36481-5
DORA PSI -
Huang C-Y, Aumonier S, Engilberge S, Eris D, Smith KML, Leonarski F, et al.
Probing ligand binding of endothiapepsin by 'temperature-resolved' macromolecular crystallography
Acta Crystallographica Section D: Structural Biology. 2022; 78: 964-974. https://doi.org/10.1107/S205979832200612X
DORA PSI -
Moreno-Chicano T, Carey LM, Axford D, Beale JH, Doak RB, Duyvesteyn HME, et al.
Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature
IUCrJ. 2022; 9(5): 610-624. https://doi.org/10.1107/S2052252522006418
DORA PSI -
Beale JH, Marsh ME
Optimizing the growth of endothiapepsin crystals for serial crystallography experiments
Journal of Visualized Experiments. 2021; 168: e61896 (30 pp.). https://doi.org/10.3791/61896
DORA PSI -
Butryn A, Simon PS, Aller P, Hinchliffe P, Massad RN, Leen G, et al.
An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography
Nature Communications. 2021; 12(1): 4461 (7 pp.). https://doi.org/10.1038/s41467-021-24757-7
DORA PSI -
Martiel I, Beale JH, Karpik A, Huang C-Y, Vera L, Olieric N, et al.
Versatile microporous polymer-based supports for serial macromolecular crystallography
Acta Crystallographica Section D: Structural Biology. 2021; 77(9): 1153-1167. https://doi.org/10.1107/S2059798321007324
DORA PSI -
Beale JH
Macromolecular X-ray crystallography: soon to be a road less travelled?
Acta Crystallographica Section D: Structural Biology. 2020; 76(5): 400-405. https://doi.org/10.1107/S2059798320004660
DORA PSI -
Pfanzagl V, Beale JH, Michlits H, Schmidt D, Gabler T, Obinger C, et al.
X-ray-induced photoreduction of heme metal centers rapidly induces active-site perturbations in a protein-independent manner
Journal of Biological Chemistry. 2020; 295(39): 13488-13501. https://doi.org/10.1074/jbc.ra120.014087
DORA PSI